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hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNAdamage

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 92-102 doi: 10.1007/s11705-021-2063-9

摘要： Mitochondrial DNA has a special structure that is prone to damage resulting in many serious diseases, such as genetic diseases and cancers. Therefore, the rapid and specific monitoring of mitochondrial DNA damage is urgently needed for biological recognition. Herein, we constructed an in situ hydrophobic environment-triggering reactive fluorescence probe named MBI-CN. The fluorophore was 2-styrene-1H-benzo[d]imidazole, and malononitrile was introduced as a core into a molecule to initiate the hydrolysis reaction in the specific environment containing damaged mitochondrial DNA. In this design, MBI-CN conjugates to mitochondrial DNA without causing additional damages. Thus, MBI-CN can be hydrolyzed to generate MBI-CHO in an in situ hydrophobic environment with mitochondrial DNA damage. Meanwhile, MBI-CHO immediately emitted a significative fluorescence signal changes at 437 and 553 nm within 25 s for the damaged mitochondria DNA. Give that the specific and rapid response of MBI-CN does not cause additional damages to mitochondrial DNA, it is a potentially effective detection tool for the real-time monitoring of mitochondrial DNA damage during cell apoptosis and initial assessment of cell apoptosis.

关键词： hydrolysis reaction     mitochondrial DNA damage     in situ hydrophobic environment trigger     fluorescence probe     apoptosis    

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Non-genetic mechanisms of diabetic nephropathy

null

《医学前沿（英文）》 2017年 第11卷 第3期   页码 319-332 doi: 10.1007/s11684-017-0569-9

摘要：

Diabetic nephropathy (DN) is one of the most common microvascular complications in diabetes mellitus patients and is characterized by thickened glomerular basement membrane, increased extracellular matrix formation, and podocyte loss. These phenomena lead to proteinuria and altered glomerular filtration rate, that is, the rate initially increases but progressively decreases. DN has become the leading cause of end-stage renal disease. Its prevalence shows a rapid growth trend and causes heavy social and economic burden in many countries. However, this disease is multifactorial, and its mechanism is poorly understood due to the complex pathogenesis of DN. In this review, we highlight the new molecular insights about the pathogenesis of DN from the aspects of immune inflammation response, epithelial–mesenchymal transition, apoptosis and mitochondrial damage, epigenetics, and podocyte–endothelial communication. This work offers groundwork for understanding the initiation and progression of DN, as well as provides ideas for developing new prevention and treatment measures.

关键词： diabetic nephropathy     immune inflammatory response     epithelial–mesenchymal transition     apoptosis     mitochondrial damage     epigenetics     podocyte–endothelial communication    

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Serum mitochondrial tsRNA serves as a novel biomarker for hepatocarcinoma diagnosis

《医学前沿（英文）》 2022年 第16卷 第2期   页码 216-226 doi: 10.1007/s11684-022-0920-7

摘要： Hepatocellular carcinoma (HCC), which makes up the majority of liver cancer, is induced by the infection of hepatitis B/C virus. Biomarkers are needed to facilitate the early detection of HCC, which is often diagnosed too late for effective therapy. The tRNA-derived small RNAs (tsRNAs) play vital roles in tumorigenesis and are stable in circulation. However, the diagnostic values and biological functions of circulating tsRNAs, especially for HCC, are still unknown. In this study, we first utilized RNA sequencing followed by quantitative reverse-transcription PCR to analyze tsRNA signatures in HCC serum. We identified tRF-Gln-TTG-006, which was remarkably upregulated in HCC serum (training cohort: 24 HCC patients vs. 24 healthy controls). In the validation stage, we found that tRF-Gln-TTG-006 signature could distinguish HCC cases from healthy subjects with high sensitivity (80.4%) and specificity (79.4%) even in the early stage (Stage I: sensitivity, 79.0%; specificity, 74.8%; 155 healthy controls vs. 153 HCC patients from two cohorts). Moreover, in vitro studies indicated that circulating tRF-Gln-TTG-006 was released from tumor cells, and its biological function was predicted by bioinformatics assay and validated by colony formation and apoptosis assays. In summary, our study demonstrated that serum tsRNA signature may serve as a novel biomarker of HCC.

关键词： tsRNA     biomarker     hepatocarcinoma    

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Conserved gene arrangement in the mitochondrial genomes of barklouse families Stenopsocidae and Psocidae

Xiaochen LIU, Hu LI, Yao CAI, Fan SONG, John-James WILSON, Wanzhi CAI

《农业科学与工程前沿（英文）》 2017年 第4卷 第3期   页码 358-365 doi: 10.15302/J-FASE-2017158

摘要： Substantial variation in gene organization and arrangement has been reported for sequenced mitochondrial (mt) genomes from the suborders of the insect order Psocoptera. In this study we sequenced the complete mt genome of , the first representative of the family Stenopsocidae from the suborder Psocomorpha. Relative to the ancestral pattern, rearrangements of a protein-coding gene ( ) and five tRNA genes ( , , , , ) were found. This pattern was similar to that of two barklice from the family Psocidae, with the exception of the translocation of , and Based on comparisons of pairwise breakpoint distances of gene rearrangements, gene number and chromosome number, it was concluded that mt genomes of Stenopsocidae and Psocidae share a relatively conserved pattern of gene rearrangements; mt genomes within the Psocomorpha have been generally stable over long evolutionary history; and mt gene rearrangement has been substantially faster in the booklice (suborder Troctomorpha) than in the barklice (suborders Trogiomorpha and Psocomorpha). It is speculated that the change of life history and persistence of unusual reproductive systems with maternal inheritance contributed to the contrasting rates in mt genome evolution between the barklice and booklice.

关键词： gene rearrangement     mitochondrial genome     Psocoptera     Stenopsocidae     TDRL model    

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N-Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probes

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

《化学科学与工程前沿（英文）》 2022年 第16卷 第1期   页码 64-71 doi: 10.1007/s11705-021-2048-8

摘要： Heterocyclic compound quinoline and its derivatives exist in natural compounds and have a broad spectrum of biological activity. They play an important role in the design of new structural entities for medical applications. Similarly, indoles and their derivatives are found widely in nature. Amino acids, alkaloids and auxin are all derivatives of indoles, as are dyes, and their condensation with aldehydes makes it easy to construct reaction sites for nucleophilic addition agents. In this work, we combine these two groups organically to construct a rapid response site (within 30 s) for H S, and at the same time, a ratiometric fluorescence response is presented throughout the process of H S detection. As such, the lower detection limit can reach 55.7 nmol/L for H S. In addition, cell imaging shows that this probe can be used for the mitochondrial targeted detection of endogenous and exogenous H S. Finally, this probe application was verified by imaging H S in nude mice.

关键词： heterocyclic compound     hydrogen sulfide     ratiometric     mitochondrial targeted    

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Berberine alleviates myocardial diastolic dysfunction by modulating Drp1-mediated mitochondrial fission

《医学前沿（英文）》 doi: 10.1007/s11684-023-0983-0

摘要： Berberine alleviates myocardial diastolic dysfunction by modulating Drp1-mediated mitochondrial fission and Ca homeostasis in a murine model of HFpEF

关键词： mitochondrial fission Ca     Berberine alleviates myocardial     murine model HFpEF    

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NRTIs’ effect on the sequence of mitochondrial DNA HV 2 in HIV infected patients

Ya-Song WU MD, PhD, Xin-Yue CHEN MD, Ying SHI PhD, Hao WU MD, De-Xi CHEN MD, PhD, Yu SUN MD, Fu-Jie ZHANG MD,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 177-184 doi: 10.1007/s11684-010-0038-1

摘要： Potent combination antiretroviral therapy (cART) has significantly improved the life expectancy of people living with human immunodeficiency virus (HIV), but it has many side effects such as lipodystrophy (LD), hepatic steatosis, and lactic acidosis. Nucleoside reverse transcriptase inhibitors (NRTIs) could damage the mitochondria by inhibiting the human DNA polymerase gamma, leading to mtDNA deletion. However, it remains uncertain whether NRTIs could induce the hypervariable region (HV) mutations of the D loop of mitochondria in Chinese HIV/AIDS patients, and whether that effect is different between individuals with and without LD. Hereby, 30 Chinese AIDS patients who were receiving antiretroviral drugs were recruited, among which 16 had symptomatic LD and 14 did not. Blood samples were collected prior to and after 96 weeks of treatment. Total DNA was extracted from peripheral blood mononuclear cells (PBMCs). Fragments of 728 bp in length containing HV 2 were amplified by standard polymerase chain reaction (PCR). Direct DNA-sequencing analysis techniques were used to detect mitochondrial sequence variants between paired longitudinal samples. Alterations were compared with the revised Cambridge Reference Sequence (rCRS) to determine mutation or polymorphism. Results showed that two years after ART, totally seven cases exhibited sequence variations, five individuals showed 73€A→G revised variation (two with and three without LD), while two cases of LD were found to have other nucleotide alterations. There was no new alteration in individuals without LD. In conclusion, NRTIs could induce mutation of mtDNA HV 2, which might contribute to the development of LD.

关键词： nucleoside reverse transcriptase inhibitors     human immunodeficiency virus     mitochondrial DNA     D loop     mutation    

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Association of gene variants with juvenile amyotrophic lateral sclerosis

《医学前沿（英文）》 doi: 10.1007/s11684-023-1005-y

摘要： Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive degeneration of motor neurons, and it demonstrates high clinical heterogeneity and complex genetic architecture. A variation within TRMT2B (c.1356G>T; p.K452N) was identified to be associated with ALS in a family comprising two patients with juvenile ALS (JALS). Two missense variations and one splicing variation were identified in 10 patients with ALS in a cohort with 910 patients with ALS, and three more variants were identified in a public ALS database including 3317 patients with ALS. A decreased number of mitochondria, swollen mitochondria, lower expression of ND1, decreased mitochondrial complex I activities, lower mitochondrial aerobic respiration, and a high level of ROS were observed functionally in patient-originated lymphoblastoid cell lines and TRMT2B interfering HEK293 cells. Further, TRMT2B variations overexpression cells also displayed decreased ND1. In conclusion, a novel JALS-associated gene called TRMT2B was identified, thus broadening the clinical and genetic spectrum of ALS.

关键词： TRMT2B     amyotrophic lateral sclerosis     mitochondrial complex I     tRNA methylation     reactive oxygen species    

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Windborne debris damage prediction analysis

Fangfang SONG, Jinping OU,

《结构与土木工程前沿（英文）》 2010年 第4卷 第3期   页码 326-330 doi: 10.1007/s11709-010-0067-5

摘要： Windborne debris is one of the most important causes of the envelop destruction according to the post-damage investigations. The problem of windborne debris damage could be summarized as three parts, including windborne debris risk analysis, debris flying trajectories, and impact resistance of envelope analysis. The method of debris distribution is developed. The flying trajectories of compact and plate-like debris are solved by using a numerical method according to the different aerodynamic characteristics. The impact resistance of the envelopes is also analyzed. Besides, the process of windborne debris damage analysis is described in detail. An example of industrial building is given to demonstrate the whole method by using the observed data of typhoon Chanchu (2006). The method developed in this paper could be applied to risk assessment of windborne debris for structures in wind hazard.

关键词： typhoon     windborne debris     structural envelopes     damage estimation    

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Corrosion damage assessment and monitoring of large steel space structures

Bo CHEN, You-Lin XU, Weilian QU,

《结构与土木工程前沿（英文）》 2010年 第4卷 第3期   页码 354-369 doi: 10.1007/s11709-010-0088-0

摘要： Large steel space structures, when exposed to a harsh corrosive environment, are inevitably subjected to atmospheric corrosion and stress corrosion cracking. This paper proposes a framework for assessing the corrosion damage of large steel space structures subjected to both stress corrosion cracking and atmospheric corrosion. The empirical model for estimating atmospheric corrosion based on measured information is briefly introduced. The proposed framework is applied to a real large steel space structure built in the southern coastal area in China to assess its corrosion damage and investigate the effects of atmospheric corrosion on stress corrosion cracking. Based on the results, the conceptual design of the corrosion monitoring system of large steel space structures is finally conducted as the first step for a real corrosion monitoring system.

关键词： large steel space structure     atmospheric corrosion     stress corrosion cracking     corrosion damage     damage assessment     monitoring system    

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Detection of damage locations and damage steps in pile foundations using acoustic emissions with deep

Alipujiang JIERULA, Tae-Min OH, Shuhong WANG, Joon-Hyun LEE, Hyunwoo KIM, Jong-Won LEE

《结构与土木工程前沿（英文）》 2021年 第15卷 第2期   页码 318-332 doi: 10.1007/s11709-021-0715-y

摘要： The aim of this study is to propose a new detection method for determining the damage locations in pile foundations based on deep learning using acoustic emission data. First, the damage location is simulated using a back propagation neural network deep learning model with an acoustic emission data set acquired from pile hit experiments. In particular, the damage location is identified using two parameters: the pile location ( ) and the distance from the pile cap ( ). This study investigates the influences of various acoustic emission parameters, numbers of sensors, sensor installation locations, and the time difference on the prediction accuracy of and . In addition, correlations between the damage location and acoustic emission parameters are investigated. Second, the damage step condition is determined using a classification model with an acoustic emission data set acquired from uniaxial compressive strength experiments. Finally, a new damage detection and evaluation method for pile foundations is proposed. This new method is capable of continuously detecting and evaluating the damage of pile foundations in service.

关键词： pile foundations     damage location     acoustic emission     deep learning     damage step    

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Creep life assessment of aero-engine recuperator based on continuum damage mechanics approach

《机械工程前沿（英文）》 2022年 第17卷 第4期 doi: 10.1007/s11465-022-0702-6

摘要： The creep life of an aeroengine recuperator is investigated in terms of continuum damage mechanics by using finite element simulations. The effects of the manifold wall thickness and creep properties of brazing filler metal on the operating life of the recuperator are analyzed. Results show that the crack initiates from the brazing filler metal located on the outer surface of the manifold with the wall thickness of 2 mm and propagates throughout the whole region of the brazing filler metal when the creep time reaches 34900 h. The creep life of the recuperator meets the requirement of 40000 h continuous operation when the wall thickness increases to 3.5 mm, but its total weight increases by 15%. Decreasing the minimum creep strain rate with the enhancement of the creep strength of the brazing filler metal presents an obvious effect on the creep life of the recuperator. At the same stress level, the creep rupture time of the recuperator is enhanced by 13 times if the mismatch between the minimum creep rate of the filler and base metal is reduced by 20%.

关键词： creep     life assessment     brazed joint     continuum damage mechanics     aeroengine recuperator    

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Bacterial inactivation, DNA damage, and faster ATP degradation induced by ultraviolet disinfection

Chao Yang, Wenjun Sun, Xiuwei Ao

《环境科学与工程前沿（英文）》 2020年 第14卷 第1期 doi: 10.1007/s11783-019-1192-6

摘要： • Long amplicon is more effective to test DNA damage induced by UV. • ATP in bacteria does not degrade instantly but does eventually after UV exposure. • After medium pressure UV exposure, ATP degraded faster. The efficacy of ultraviolet (UV) disinfection has been validated in numerous studies by using culture-based methods. However, the discovery of viable but non-culturable bacteria has necessitated the investigation of UV disinfection based on bacterial viability parameters. We used quantitative polymerase chain reaction (qPCR) to investigate DNA damage and evaluated adenosine triphosphate (ATP) to indicate bacterial viability. The results of qPCR effectively showed the DNA damage induced by UV when using longer gene amplicons, in that sufficiently long amplicons of both 16S and gadA indicated that the UV induced DNA damages. The copy concentrations of the long amplicons of 16S and gadA decreased by 2.38 log/mL and 1.88 log/mL, respectively, after exposure to 40 mJ/cm2 low-pressure UV. After UV exposure, the ATP level in the bacteria did not decrease instantly. Instead it decreased gradually at a rate that was positively related to the UV fluence. For low-pressure UV, this rate of decrease was slow, but for medium pressure UV, this rate of decrease was relatively high when the UV fluence reached 40 mJ/cm2. At the same UV fluence, the ATP level in the bacteria decreased at a faster rate after exposure to medium-pressure UV.

关键词： UV disinfection     DNA damage     qPCR     ATP    

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The damage evolution behavior of polypropylene fiber reinforced concrete subjected to sulfate attack

Ninghui LIANG; Jinwang MAO; Ru YAN; Xinrong LIU; Xiaohan ZHOU

《结构与土木工程前沿（英文）》 2022年 第16卷 第3期   页码 316-328 doi: 10.1007/s11709-022-0810-8

摘要： To study the damage evolution behavior of polypropylene fiber reinforced concrete (PFRC) subjected to sulfate attack, a uniaxial compression test was carried out based on acoustic emission (AE). The effect of sulfate attack relative to time and fiber hybridization were analyzed and the compression damage factor was calculated using a mathematical model. The changes to AE ringing counts during the compression could be divided into compaction, elastic, and AE signal hyperactivity stages. In the initial stage of sulfate attack, the concrete micropores and microcracks were compacted gradually under external load and a corrosion products filling effect, and this corresponded with detection of few AE signals and with concrete compression strength enhancement. With increasing sulfate attack time, AE activity decreased. The cumulative AE ringing counts of PFRC at all corrosion ages were much higher than those for plain concrete. PFRC could still produce AE signals after peak load due to drawing effect of polypropylene fiber. After 150 d of sulfate attack, the cumulative AE ringing counts of plain concrete went down by about an order of magnitude, while that for PFRC remained at a high level. The initial damage factor of hybrid PFRC was −0.042 and −0.056 respectively after 150 d of corrosion, indicating that the advantage of hybrid polypropylene fiber was more obvious than plain concrete and single-doped PFRC. Based on a deterioration equation, the corrosion resistance coefficient of hybrid PFRC would be less than 0.75 after 42 drying−wetting sulfate attack cycles, which was 40% longer than that of plain concrete.

关键词： polypropylene fiber reinforced concrete     sulfate attack     damage evolution behavior     acoustic emission     damage factor    

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Spectral element modeling based structure piezoelectric impedance computation and damage identification

Zhigang GUO, Zhi SUN

《结构与土木工程前沿（英文）》 2011年 第5卷 第4期   页码 458-464 doi: 10.1007/s11709-011-0133-7

摘要： This paper presents a numerical simulation study on electromechanical impedance technique for structural damage identification. The basic principle of impedance based damage detection is structural impedance will vary with the occurrence and development of structural damage, which can be measured from electromechanical admittance curves acquired from PZT patches. Therefore, structure damage can be identified from the electromechanical admittance measurements. In this study, a model based method that can identify both location and severity of structural damage through the minimization of the deviations between structural impedance curves and numerically computed response is developed. The numerical model is set up using the spectral element method, which is promised to be of high numerical efficiency and computational accuracy in the high frequency range. An optimization procedure is then formulated to estimate the property change of structural elements from the electric admittance measurement of PZT patches. A case study on a pin-pin bar is conducted to investigate the feasibility of the proposed method. The results show that the presented method can accurately identify bar damage location and severity even when the measurements are polluted by 5% noise.

关键词： PZT     piezoelectric impedance     optimization     spectral element     damage identification    

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标题 作者 时间 类型 操作

hydrophobic environment triggering reactive fluorescence probe to real-time monitor mitochondrial DNAdamage

期刊论文

Non-genetic mechanisms of diabetic nephropathy

null

期刊论文

Serum mitochondrial tsRNA serves as a novel biomarker for hepatocarcinoma diagnosis

期刊论文

Conserved gene arrangement in the mitochondrial genomes of barklouse families Stenopsocidae and Psocidae

Xiaochen LIU, Hu LI, Yao CAI, Fan SONG, John-James WILSON, Wanzhi CAI

期刊论文

N-Positive ion activated rapid addition and mitochondrial targeting ratiometric fluorescent probes

Yan Shi, Fangjun Huo, Yongkang Yue, Caixia Yin

期刊论文

Berberine alleviates myocardial diastolic dysfunction by modulating Drp1-mediated mitochondrial fission

期刊论文

NRTIs’ effect on the sequence of mitochondrial DNA HV 2 in HIV infected patients

Ya-Song WU MD, PhD, Xin-Yue CHEN MD, Ying SHI PhD, Hao WU MD, De-Xi CHEN MD, PhD, Yu SUN MD, Fu-Jie ZHANG MD,

期刊论文

Association of gene variants with juvenile amyotrophic lateral sclerosis

期刊论文

Windborne debris damage prediction analysis

Fangfang SONG, Jinping OU,

期刊论文

Corrosion damage assessment and monitoring of large steel space structures

Bo CHEN, You-Lin XU, Weilian QU,

期刊论文

Detection of damage locations and damage steps in pile foundations using acoustic emissions with deep

Alipujiang JIERULA, Tae-Min OH, Shuhong WANG, Joon-Hyun LEE, Hyunwoo KIM, Jong-Won LEE

期刊论文

Creep life assessment of aero-engine recuperator based on continuum damage mechanics approach

期刊论文

Bacterial inactivation, DNA damage, and faster ATP degradation induced by ultraviolet disinfection

Chao Yang, Wenjun Sun, Xiuwei Ao

期刊论文

The damage evolution behavior of polypropylene fiber reinforced concrete subjected to sulfate attack

Ninghui LIANG; Jinwang MAO; Ru YAN; Xinrong LIU; Xiaohan ZHOU

期刊论文

Spectral element modeling based structure piezoelectric impedance computation and damage identification

Zhigang GUO, Zhi SUN

期刊论文